出品公司: | Promega |
---|---|
载体名称: | pGL4.35[luc2P/9XGAL4UAS/Hygro] |
质粒类型: | 荧光素酶载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 6184 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
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pGL4.35[luc2P/9XGAL4UAS/Hygro]载体质粒基本信息
pGL4.35[luc2P/9XGAL4UAS/Hygro]质粒图谱载体图谱和pGL4.35[luc2P/9XGAL4UAS/Hygro]载体序列质粒序列多克隆位点信息
pGL4.35[luc2P/9XGAL4UAS/Hygro]质粒载体简介
pGL4.35[luc2P/9XGAL4UAS/Hygro]质粒序列载体序列
LOCUS pGL4.35[luc2P 9XGAL4UAS Hygro] 6184 bp ds-DNA circular SYN 27-JUL-2013 DEFINITION Vector with a promoter and an extended Gal4 upstream activating sequence for studying cell signaling using destabilized luciferase. ACCESSION GQ229577 VERSION . KEYWORDS pGL4.35[luc2P 9XGAL4UAS Hygro] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6184) AUTHORS Promega TITLE Direct Submission JOURNAL Exported Wednesday, February 19, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6184 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" protein_bind 31..212 /bound_moiety="Gal4" /note="9X GAL4 UAS" /note="upstream activating sequence mediating Gal4-dependent induction" promoter 222..262 /note="Ad promoter" /note="adenovirus major late promoter" CDS 315..1964 /codon_start=1 /product="firefly luciferase" /note="luciferase" /note="synthetic luc2 version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" CDS 1968..2087 /codon_start=1 /product="PEST degradation sequence from mouse ornithine decarboxylase" /note="hPEST" /note="human codon-optimized" /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV" polyA_signal 2139..2260 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 2454..2811 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2662..2797 /note="SV40 ori" /note="SV40 origin of replication" CDS 2842..3879 /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK EVGRV" polyA_signal 3903..3951 /note="synthetic polyadenylation signal" rep_origin complement(4278..4866) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5066..5926) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 6031..6079 /note="synthetic polyadenylation signal" misc_feature 6093..6184 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt 61 cctccgactc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 121 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 181 ctccgaggaa ttccggagta ctgtcctccg aagacgctag cggggggcta taaaaggggg 241 tgggggcgtt cgtcctcact ctagatctgc gatctaagta agcttggcaa tccggtactg 301 ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc 361 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg 421 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt 481 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc 541 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc 601 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga 661 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga 721 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga 781 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct 841 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca 901 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt 961 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg 1021 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact 1081 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca 1141 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg 1201 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg 1261 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca 1321 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg 1381 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact 1441 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca 1501 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg 1561 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg 1621 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga 1681 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg 1741 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga 1801 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg 1861 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc 1921 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgaattct cacggcttcc 1981 ctcccgaggt ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg 2041 gcatggatag acaccctgct gcttgcgcca gcgccaggat caacgtctaa ggccgcgact 2101 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg 2161 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 2221 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 2281 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 2341 caaatgtggt aaaatcgata aggatccgtt tgcgtattgg gcgctcttcc gctgatctgc 2401 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttagctac cttctgaggc 2461 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 2521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 2581 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 2641 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 2701 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 2761 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat 2821 tcttctgaca ctagcgccac catgaagaag cccgaactca ccgctaccag cgttgaaaaa 2881 tttctcatcg agaagttcga cagtgtgagc gacctgatgc agttgtcgga gggcgaagag 2941 agccgagcct tcagcttcga tgtcggcgga cgcggctatg tactgcgggt gaatagctgc 3001 gctgatggct tctacaaaga ccgctacgtg taccgccact tcgccagcgc tgcactaccc 3061 atccccgaag tgttggacat cggcgagttc agcgagagcc tgacatactg catcagtaga 3121 cgcgcccaag gcgttactct ccaagacctc cccgaaacag agctgcctgc tgtgttacag 3181 cctgtcgccg aagctatgga tgctattgcc gccgccgacc tcagtcaaac cagcggcttc 3241 ggcccattcg ggccccaagg catcggccag tacacaacct ggcgggattt catttgcgcc 3301 attgctgatc cccatgtcta ccactggcag accgtgatgg acgacaccgt gtccgccagc 3361 gtagctcaag ccctggacga actgatgctg tgggccgaag actgtcccga ggtgcgccac 3421 ctcgtccatg ccgacttcgg cagcaacaac gtcctgaccg acaacggccg catcaccgcc 3481 gtaatcgact ggtccgaagc tatgttcggg gacagtcagt acgaggtggc caacatcttc 3541 ttctggcggc cctggctggc ttgcatggag cagcagactc gctacttcga gcgccggcat 3601 cccgagctgg ccggcagccc tcgtctgcga gcctacatgc tgcgcatcgg cctggatcag 3661 ctctaccaga gcctcgtgga cggcaacttc gacgatgctg cctgggctca aggccgctgc 3721 gatgccatcg tccgcagcgg ggccggcacc gtcggtcgca cacaaatcgc tcgccggagc 3781 gcagccgtat ggaccgacgg ctgcgtcgag gtgctggccg acagcggcaa ccgccggccc 3841 agtacacgac cgcgcgctaa ggaggtaggt cgagtttaaa ctctagaacc ggtcatggcc 3901 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gttcgaacta 3961 gatgctgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 4021 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 4081 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 4681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc 5041 cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag 5101 cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga 5161 ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac 5221 cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc 5281 ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa 5341 gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac 5401 gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat 5461 gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa 5521 gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg 5581 tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg 5641 agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc 5701 cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact 5761 caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat 5821 cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg 5881 ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc 5941 aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata 6001 ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg 6061 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg 6121 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt 6181 ctct //
pGL4.35[luc2P/9XGAL4UAS/Hygro]载体图谱质粒图谱pdf版和pGL4.35[luc2P/9XGAL4UAS/Hygro]载体序列质粒序列等相关资料下载
pGL4.35[luc2P/9XGAL4UAS/Hygro]质粒载体应用举例
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